Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p157 | (1) | WCRB2014

A high concentration of polyvinylpyrrolidone in culture medium affects the number of tranzonal projections in bovine oocyte–granulosa cell complexes in long-term culture

Hirao Yuji , Somfai Tamas , Akagi Satoshi , Haraguchi Seiki , Watanabe Shinya

Introduction: A high concentration of polyvinylpyrrolidone (PVP, average molecular weight 360 000) supplementation in culture medium exerts a positive influence on the growth and morphological organization of bovine oocyte–cumulus/granulosa cell complexes in a long-term culture. The objective of the present study was to determine the effect of PVP supplementation on the number of transzonal projections (TZPs) traversing the zona pellucida from cumulus cells to the oocyte....

ra0001p106 | (1) | WCRB2014

Genome-wide DNA methylation analysis of bovine clones derived from the same donor cells

Kaneda Masahiro , Watanabe Shinya , Hirako Makoto , Akagi Satoshi , Kiefer Helene , Jouneau Luc , Martin-Magniette Marie-Laure , Balzergue Sandrine , Nagai Takashi

Introduction: What is nature versus nurture? To what extent do genetic inheritance and non-genetic factors contribute to one’s character? This is one of the oldest issues in psychology. To answer this historic question, cloned animals are good models because they have identical genetic information to the donors. Previous studies indicated that cloned animals showed different coat color patterns, noseprints and characters compared to the donors. However, no molecular work ...